Plasmid restriction digest gel worksheet: draw the double digest

An example figure from the Gel Electrophoresis generator.

A 1% agarose gel with four lanes labeled Ladder, EcoRI, HindIII and Both; blank lines beside the ladder’s ten bands, empty circles at both ends for the electrodes, and the Both lane left empty — A 1% agarose gel of a 5,000 bp plasmid cut by EcoRI, which leaves one band, by HindIII, which leaves two, and by both enzymes together. The Both lane is left empty for students to draw its bands from the two single digests. The 1 kb ladder’s ten bands have blank lines beside them for students to label, and the electrodes are empty circles for them to mark − and +.

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Answer key

Ladder: 10000, 8000, 6000, 5000, 4000, 3000, 2000, 1500, 1000, 500 bp
EcoRI: 5000 bp
HindIII: 3200, 1800 bp

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